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ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Confocal imaging of HT-29 cells using ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and HT-29 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line). Non-fluorescently stained cells was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 HeLa cells(Low Expression) were surface-stained with ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained HeLa cells was used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 HT-29 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 HeLa cells (Low Expression) were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human CD326/EPCAM mAb
Catalog No.A22486
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57676-ABf647
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-265 of human CD326/EPCAM (NP_002345.2).
SequenceQEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDESGLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEKDVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLK
Gene ID
Swiss Prot
SynonymsESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; BerEp4; EGP314; HNPCC8; LYNCH8; MOC-31; Ber-Ep4; TACSTD1
Calculated MW35kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular locationApical plasma membrane, Basolateral plasma membrane, Bicellular tight junction, Extracellular exosome, Lateral plasma membrane, Plasma membrane.

Documents

Certificate of Compliance

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Lot number

ABclonal:Immunofluorescence - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)}

Immunofluorescence - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Confocal imaging of HT-29 cells using ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and HT-29 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line). Non-fluorescently stained cells was used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 HeLa cells(Low Expression) were surface-stained with ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained HeLa cells was used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 HT-29 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486)

Flow cytometry: 1X10^6 HeLa cells (Low Expression) were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD326/EPCAM mAb (A22486, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

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