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EpCAM Rabbit pAb (A1177)

Reviews (2)Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - EpCAM Rabbit pAb (A1177)

Western blot analysis of various lysates, using EpCAM Rabbit pAb (A1177) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - EpCAM Rabbit pAb (A1177)

Immunofluorescence analysis of A431 cells using EpCAM Rabbit pAb (A1177) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - EpCAM Rabbit pAb (A1177)

Immunofluorescence analysis of HT-29 cells using EpCAM Rabbit pAb (A1177) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for EpCAM Rabbit pAb(A1177)
Review
ABclonal: review for EpCAM Rabbit pAb(A1177)

Overview

Product nameEpCAM Rabbit pAb
Catalog No.A1177
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-265 of human EpCAM (NP_002345.2).
SequenceQEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDESGLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEKDVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLK
Gene ID
Swiss Prot
SynonymsESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; BerEp4; EGP314; HNPCC8; LYNCH8; MOC-31; Ber-Ep4; TACSTD1; EpCAM
Calculated MW35kDa
Observed MW35-45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHT-29, Mouse colon, Rat colon
Cellular locationCell junction, Lateral cell membrane, Single-pass type I membrane protein, tight junction.
Customer validation

(Mouse lung tumor, Mouse adenocarcinoma)

WB(Mus musculus, Homo sapiens)

IF(Mus musculus, Homo sapiens, Mus musculus)

IHC(Mus musculus)

IHC(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - EpCAM Rabbit pAb (A1177)}

Western blot - EpCAM Rabbit pAb (A1177)

Western blot analysis of various lysates, using EpCAM Rabbit pAb (A1177) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - EpCAM Rabbit pAb (A1177)}

Immunofluorescence - EpCAM Rabbit pAb (A1177)

Immunofluorescence analysis of A431 cells using EpCAM Rabbit pAb (A1177) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - EpCAM Rabbit pAb (A1177)}

Immunofluorescence - EpCAM Rabbit pAb (A1177)

Immunofluorescence analysis of HT-29 cells using EpCAM Rabbit pAb (A1177) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

재조합 단백질 (2)

Secondary Antibodies (26)