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ABflo® 647 Rabbit anti-Human FAP mAb (A24966)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human FAP mAb (A24966)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and U-87MG cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human FAP mAb (A24966, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human FAP mAb (A24966)

Flow cytometry: 1X10^6 U-87MG cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human FAP mAb (A24966, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human FAP mAb
Catalog No.A24966
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64735-ABflo647
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 26-760 of human FAP (NP_004451.2).
SequenceLRPSRVHNSEENTMRALTLKDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEEYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD
Gene ID
Swiss Prot
SynonymsFAP; DPPIV; FAPA; FAPalpha; SIMP; prolyl endopeptidase FAP
Calculated MW27kDa/87kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Cell projection, Cell surface, Cytoplasm, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein, invadopodium membrane, lamellipodium membrane, ruffle membrane

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human FAP mAb (A24966)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human FAP mAb (A24966)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and U-87MG cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human FAP mAb (A24966, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human FAP mAb (A24966)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human FAP mAb (A24966)

Flow cytometry: 1X10^6 U-87MG cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human FAP mAb (A24966, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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