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ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A549 cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479)

Flow cytometry: 1X10^6 A549 cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479, 5 μl/Test, right).

Overview

Product nameABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb
Catalog No.A26479
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55418-ABflo700
This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. This protein has also been determined to act as a co-receptor for SARS-CoV-2 (which causes COVID-19) to infect host cells.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-644 of human CD304/Neuropilin-1 (NP_003864.5).
SequenceFRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSEFP
Gene ID
Swiss Prot
SynonymsNP1; NRP; BDCA4; CD304; VEGF165R
Calculated MW68kDa/71kDa/103kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.

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ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479)}

Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A549 cells (right) were surface-stained with ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479, 5 μl/Test, orange line) or ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479)}

Flow CytoMetry - ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479)

Flow cytometry: 1X10^6 A549 cells were surface-stained with ABflo® 700 Rabbit IgG isotype control (A25976, 5 μl/Test, left) or ABflo® 700 Rabbit anti-Human CD304/Neuropilin-1 mAb (A26479, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (6)

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Secondary Antibodies (26)