제품 > 항체 > 단일클론항체(mAb)

Neuropilin-1 Rabbit mAb (A25373)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and A549 cells (right) were surface-stained with Neuropilin-1 Rabbit mAb (A25373, 2 μg/mL, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line), followed by PE Donkey anti-rabbit Antibody staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 A549 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or Neuropilin-1 Rabbit mAb (A25373, 2 μg/mL, right).

Overview

Product name	Neuropilin-1 Rabbit mAb
Catalog No.	A25373
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55418

Background

This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. This protein has also been determined to act as a co-receptor for SARS-CoV-2 (which causes COVID-19) to infect host cells.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-644 of human Neuropilin-1 (NP_003864.5).
Sequence	FRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINFNPHFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFLFIKFVSDYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKYPNSLECTYIVFVPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWDGFPDVGPHIGRYCGQKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYSVLQSSVSEDFKCMEALGMESGEIHSDQITASSQYSTNWSAERSRLNYPENGWTPGEDSYREWIQVDLGLLRFVTAVGTQGAISKETKKKYYVKTYKIDVSSNGEDWITIKEGNKPVLFQGNTNPTDVVVAVFPKPLITRFVRIKPATWETGISMRFEVYGCKITDYPCSGMLGMVSGLISDSQITSSNQGDRNWMPENIRLVTSRSGWALPPAPHSYINEWLQIDLGEEKIVRGIIIQGGKHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFEGNNNYDTPELRTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPTTPNGNLVDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSEFP
Gene ID
Swiss Prot
Synonyms	NP1; NRP; BDCA4; CD304; VEGF165R
Calculated MW	68kDa/71kDa/103kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Secreted, Single-pass type I membrane protein, axon, cytoplasmic vesicle, cytosol, early endosome, extracellular space, glutamatergic synapse, neurofilament, neuron projection, plasma membrane, postsynaptic membrane, sorting endosome

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Neuropilin-1 Rabbit mAb images

Flow CytoMetry - Neuropilin-1 Rabbit mAb (A25373)

Flow cytometry: 1X10^6 A549 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or Neuropilin-1 Rabbit mAb (A25373, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.