제품 > 항체 > 단일클론항체(mAb)

ACAT2 Rabbit mAb (A1399)

Publication (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACAT2 Rabbit mAb (A1399)

Western blot analysis of extracts of various cell lines, using ACAT2 antibody (A1399) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - ACAT2 Rabbit mAb (A1399)

Western blot analysis of extracts of various cell lines, using ACAT2 antibody (A1399) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameACAT2 Rabbit mAb
Catalog No.A1399
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2560
The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 150-250 of human ACAT2 (Q9BWD1).
SequenceGLTDAFHNCHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRKGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPAN
Gene ID
Swiss Prot
SynonymsACAT2; acetyl-CoA acetyltransferase 2
Calculated MW41kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, SW620, HepG2, Mouse liver, Mouse brain, Mouse kidney, Rat brain, Rat kidney
Cellular locationCytoplasm
Customer validation

WB(Actinopterygii, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - ACAT2 Rabbit mAb (A1399)}

Western blot - ACAT2 Rabbit mAb (A1399)

Western blot analysis of extracts of various cell lines, using ACAT2 antibody (A1399) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - ACAT2 Rabbit mAb (A1399)}

Western blot - ACAT2 Rabbit mAb (A1399)

Western blot analysis of extracts of various cell lines, using ACAT2 antibody (A1399) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1399? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)