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ACAT2 Rabbit pAb (A7866)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACAT2 Rabbit pAb (A7866)

Western blot analysis of various lysates using ACAT2 Rabbit pAb (A7866) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Human oophoroma using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - ACAT2 Rabbit pAb (A7866)

Immunofluorescence analysis of L929 cells using ACAT2 Rabbit pAb (A7866) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameACAT2 Rabbit pAb
Catalog No.A7866
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 159-277 of human ACAT2 (NP_005882.2).
SequenceHMGITAENVAKKWQVSREDQDKVAVLSQNRTENAQKAGHFDKEIVPVLVSTRKGLIEVKTDEFPRHGSNIEAMSKLKPYFLTDGTGTVTPANASGINDGAAAVVLMKKSEADKRGLTPL
Gene ID
Swiss Prot
SynonymsACAT2
Calculated MW41kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBT-474, MCF7, HeLa, HepG2, PC-12, Mouse liver
Cellular locationCytoplasm.

Documents

Certificate of Compliance

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ABclonal:Western blot - ACAT2 Rabbit pAb (A7866)}

Western blot - ACAT2 Rabbit pAb (A7866)

Western blot analysis of various lysates using ACAT2 Rabbit pAb (A7866) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)}

Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)}

Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)}

Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Human oophoroma using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACAT2 Rabbit pAb (A7866)}

Immunohistochemistry - ACAT2 Rabbit pAb (A7866)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ACAT2 Rabbit pAb (A7866) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - ACAT2 Rabbit pAb (A7866)}

Immunofluorescence - ACAT2 Rabbit pAb (A7866)

Immunofluorescence analysis of L929 cells using ACAT2 Rabbit pAb (A7866) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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