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ACLY Rabbit mAb (A22273)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACLY Rabbit mAb (A22273)

Western blot analysis of various lysates, using ACLY Rabbit mAb (A22273) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded mouse colon tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded human esophagus tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded rat brain tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded mouse brain tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded rat lung tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameACLY Rabbit mAb
Catalog No.A22273
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56113
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_001087.2).
SequenceFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM
Gene ID
Swiss Prot
SynonymsACL; ATPCL; CLATP; ACLY
Calculated MW121kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:1000 - 1:5000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, HepG2, NIH/3T3, Mouse lung, Mouse testis, C6
Cellular locationCytoplasm, Cytosol

Documents

Certificate of Compliance

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ABclonal:Western blot - ACLY Rabbit mAb (A22273)}

Western blot - ACLY Rabbit mAb (A22273)

Western blot analysis of various lysates, using ACLY Rabbit mAb (A22273) at1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)}

Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded mouse colon tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)}

Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded human esophagus tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)}

Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded rat brain tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)}

Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded mouse brain tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACLY Rabbit mAb (A22273)}

Immunohistochemistry - ACLY Rabbit mAb (A22273)

Immunohistochemistry analysis of ACLY in paraffin-embedded rat lung tissue using ACLY Rabbit mAb (A22273) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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