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ACLY Rabbit pAb (A15251)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACLY Rabbit pAb (A15251)

Western blot analysis of various lysates using ACLY Rabbit pAb (A15251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - ACLY Rabbit pAb (A15251)

Western blot analysis of various lysates using ACLY Rabbit pAb (A15251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded rat kidney using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded rat ovary using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of HeLa cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of HepG2 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of MCF7 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of NIH/3T3 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of PC-12 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameACLY Rabbit pAb
Catalog No.A15251
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 752-1101 of human ACLY (NP_001087.2).
SequenceFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM
Gene ID
Swiss Prot
SynonymsACL; ATPCL; CLATP; ACLY
Calculated MW121kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, HeLa, Rat lung, Rat liver, Mouse lung, Mouse liver, Mouse brain
Cellular locationCytoplasm

Documents

Certificate of Compliance

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ABclonal:Western blot - ACLY Rabbit pAb (A15251)}

Western blot - ACLY Rabbit pAb (A15251)

Western blot analysis of various lysates using ACLY Rabbit pAb (A15251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - ACLY Rabbit pAb (A15251)}

Western blot - ACLY Rabbit pAb (A15251)

Western blot analysis of various lysates using ACLY Rabbit pAb (A15251) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)}

Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)}

Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)}

Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded rat kidney using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ACLY Rabbit pAb (A15251)}

Immunohistochemistry - ACLY Rabbit pAb (A15251)

Immunohistochemistry analysis of paraffin-embedded rat ovary using ACLY Rabbit pAb (A15251) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)}

Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of HeLa cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)}

Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of HepG2 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)}

Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of MCF7 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)}

Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of NIH/3T3 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACLY Rabbit pAb (A15251)}

Immunofluorescence - ACLY Rabbit pAb (A15251)

Immunofluorescence analysis of PC-12 cells using ACLY Rabbit pAb (A15251) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (4)

Secondary Antibodies (26)