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Phospho-ACLY-S455 Rabbit mAb (AP1474)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Western blot analysis of lysates from HeLa cells using Phospho-ACLY-S455 Rabbit mAb (AP1474) at 1:1000 dilution. HeLa cells were treated by Insulin (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-ACLY-S455 Rabbit mAb (AP1474) at 1:1000 dilution. NIH/3T3 cells were treated by Insulin (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Western blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit mAb (AP1474) at 1:1000 dilution. C6 cells were treated by Insulin (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product namePhospho-ACLY-S455 Rabbit mAb
Catalog No.AP1474
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63942
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440, 000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenA synthetic phosphorylated peptide around S455 of human ACLY (NP_001087.2).
SequenceTASFS
Gene ID
Swiss Prot
SynonymsACL; ATPCL; CLATP; Phospho-ACLY-S455
Calculated MW121kDa
Observed MW125kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesC6 treated by Insulin, HeLa treated by Insulin, NIH/3T3 treated by Insulin
Cellular locationCytoplasm

Documents

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ABclonal:Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)}

Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Western blot analysis of lysates from HeLa cells using Phospho-ACLY-S455 Rabbit mAb (AP1474) at 1:1000 dilution. HeLa cells were treated by Insulin (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)}

Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Western blot analysis of lysates from NIH/3T3 cells using Phospho-ACLY-S455 Rabbit mAb (AP1474) at 1:1000 dilution. NIH/3T3 cells were treated by Insulin (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)}

Western blot - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Western blot analysis of lysates from C6 cells using Phospho-ACLY-S455 Rabbit mAb (AP1474) at 1:1000 dilution. C6 cells were treated by Insulin (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)}

Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)}

Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)}

Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)}

Immunohistochemistry - Phospho-ACLY-S455 Rabbit mAb (AP1474)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Phospho-ACLY-S455 Rabbit mAb (AP1474) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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