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ACSL3 Rabbit pAb (A11679)

Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACSL3 Rabbit pAb (A11679)

Western blot analysis of various lysates using ACSL3 Rabbit pAb (A11679) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Human stomach using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Rat heart using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameACSL3 Rabbit pAb
Catalog No.A11679
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 621-720 of human ACSL3 (NP_004448.2).
SequenceVIGFVVPNQKELTELARKKGLKGTWEELCNSCEMENEVLKVLSEAAISASLEKFEIPVKIRLSPEPWTPETGLVTDAFKLKRKELKTHYQADIERMYGRK
Gene ID
Swiss Prot
SynonymsACS3; FACL3; LACS3; LACS 3; PRO2194; ACSL3
Calculated MW80kDa
Observed MW76kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesK-562, HeLa
Cellular locationEndoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein.
Customer validation

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

qRT-PCR(Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - ACSL3 Rabbit pAb (A11679)}

Western blot - ACSL3 Rabbit pAb (A11679)

Western blot analysis of various lysates using ACSL3 Rabbit pAb (A11679) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)}

Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Human mammary cancer using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)}

Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Human stomach using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)}

Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Rat heart using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACSL3 Rabbit pAb (A11679)}

Immunohistochemistry - ACSL3 Rabbit pAb (A11679)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using ACSL3 Rabbit pAb (A11679) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)