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ALDH1A1 Rabbit PolymAb® (A22531PM)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ALDH1A1 Rabbit PolymAb® (A22531PM)

Western blot analysis of various lysates, using ALDH1A1 Rabbit PolymAb® (A22531PM) at 1:50000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): THP-1
Exposure time: 20s.

ABclonal:Western blot - ALDH1A1 Rabbit PolymAb® (A22531PM)

Western blot analysis of lysates from rat lung, using ALDH1A1 Rabbit PolymAb® (A22531PM) at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.

ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunofluorescence analysis of A-549 cells using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:800 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunofluorescence analysis of HepG2 cells using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:800 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameALDH1A1 Rabbit PolymAb®
Catalog No.A22531PM
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 250-350 of human ALDH1A1 Rabbit PolymAb® (NP_000680.2).
SequenceVGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQ
Gene ID
Swiss Prot
SynonymsALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Calculated MW55kDa
Observed MW55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:60000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA549, Hep G2, Mouse liver, Mouse testis, Rat lung
Cellular locationCytoplasm.
ABclonal:Western blot - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Western blot - ALDH1A1 Rabbit PolymAb® (A22531PM)

Western blot analysis of various lysates, using ALDH1A1 Rabbit PolymAb® (A22531PM) at 1:50000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): THP-1
Exposure time: 20s.
ABclonal:Western blot - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Western blot - ALDH1A1 Rabbit PolymAb® (A22531PM)

Western blot analysis of lysates from rat lung, using ALDH1A1 Rabbit PolymAb® (A22531PM) at 1:50000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.
ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Immunohistochemistry - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Immunofluorescence - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunofluorescence analysis of A-549 cells using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:800 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ALDH1A1 Rabbit PolymAb® (A22531PM)}

Immunofluorescence - ALDH1A1 Rabbit PolymAb® (A22531PM)

Immunofluorescence analysis of HepG2 cells using ALDH1A1 Rabbit PolymAb® (A22531PM) at dilution of 1:800 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

ELISA 키트 (1)

Secondary Antibodies (26)