Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of lysates from ALPK1 knockout (KO) 293T and ALPK1 knockout (KO) 293T transfected with mouse ALPK1 using ALPK1 Rabbit mAb (A24046) at 1:7000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using ALPK1 Rabbit mAb (A24046) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
| Product name | ALPK1 Rabbit mAb |
|---|---|
| Catalog No. | A24046 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC63314 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 230-478 of mouse ALPK1（NP_082084.1）. |
|---|---|
| Sequence | PDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHHLLSASEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSVHLCEAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLTTVHSRLHGETDAVRAARQLCSEAVGKLYTFSTSPTSQDREGLSQEIMSLISQVKGHLRVQSFPNLDACSYVPESFKCGLDRLILHGHVDFQQILETYSQHHTSVCEVFESTCGNSK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Alpha-kinase 1; ALPK1 |
| Calculated MW | 139kDa |
| Observed MW | 150kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | 293T cells transfected with Mouse ALPK1 |
| Cellular location | Cytoplasm, cytoskeleton. |
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* For research use only. Not for therapeutic or diagnostic purposes.
