제품 > 항체 > 단일클론항체(mAb)

APC Rabbit anti-Human CD228/MFI2 mAb (A25625)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 HUVEC cells (Low Expression, left) and A-375 cells (right) were surface-stained with APC Rabbit anti-Human CD228/MFI2 mAb (A25625, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, left). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 A-375 cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD228/MFI2 mAb (A25625, 5 μl/Test, right).

Overview

Product name	APC Rabbit anti-Human CD228/MFI2 mAb
Catalog No.	A25625
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66603-APC

Background

The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-708 of human CD228/MFI2 (NP_005920.2).
Sequence	GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQ
Gene ID
Swiss Prot
Synonyms	MTf; MFI2; MTF1; CD228; MAP97
Calculated MW	32kDa/80kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, gPI-anchor, lipid-anchor

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증CDs

APC Rabbit anti-Human CD228/MFI2 mAb images

Flow CytoMetry - APC Rabbit anti-Human CD228/MFI2 mAb (A25625)

Flow cytometry: 1X10^6 A-375 cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD228/MFI2 mAb (A25625, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.