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MFI2 Rabbit mAb (A25456)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - MFI2 Rabbit mAb (A25456)

Western blot analysis of lysates from A-375 cells using MFI2 Rabbit mAb (A25456) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - MFI2 Rabbit mAb (A25456)

Western blot analysis of lysates from Rat uterus using MFI2 Rabbit mAb (A25456) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Flow CytoMetry - MFI2 Rabbit mAb (A25456)

Flow cytometry: 1X10^6 HUVEC cells (Low Expression, left) and A-375 cells (right) were surface-stained with MFI2 Rabbit mAb (A25456, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - MFI2 Rabbit mAb (A25456)

Flow cytometry: 1X10^6 A-375 cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or MFI2 Rabbit mAb (A25456, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

Overview

Product nameMFI2 Rabbit mAb
Catalog No.A25456
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC66603
The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-708 of human MFI2 (NP_005920.2).
SequenceGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQ
Gene ID
Swiss Prot
SynonymsMTf; MFI2; MTF1; CD228; MAP97
Calculated MW32kDa/80kDa
Observed MW80-90kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • FC 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Flow Cytometry    
Positive samplesA-375, Rat uterus
Cellular locationCell membrane, gPI-anchor, lipid-anchor.

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ABclonal:Western blot - MFI2 Rabbit mAb (A25456)}

Western blot - MFI2 Rabbit mAb (A25456)

Western blot analysis of lysates from A-375 cells using MFI2 Rabbit mAb (A25456) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Western blot - MFI2 Rabbit mAb (A25456)}

Western blot - MFI2 Rabbit mAb (A25456)

Western blot analysis of lysates from Rat uterus using MFI2 Rabbit mAb (A25456) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Flow CytoMetry - MFI2 Rabbit mAb (A25456)}

Flow CytoMetry - MFI2 Rabbit mAb (A25456)

Flow cytometry: 1X10^6 HUVEC cells (Low Expression, left) and A-375 cells (right) were surface-stained with MFI2 Rabbit mAb (A25456, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - MFI2 Rabbit mAb (A25456)}

Flow CytoMetry - MFI2 Rabbit mAb (A25456)

Flow cytometry: 1X10^6 A-375 cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or MFI2 Rabbit mAb (A25456, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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