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APEH Rabbit pAb (A13467)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - APEH Rabbit pAb (A13467)

Western blot analysis of extracts of various cell lines, using APEH antibody (A13467) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - APEH Rabbit pAb (A13467)

Immunofluorescence analysis of HeLa cells using APEH antibody (A13467). Blue: DAPI for nuclear staining.

Overview

Product nameAPEH Rabbit pAb
Catalog No.A13467
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
SequenceMERQVLLSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVLRKAGGTGPGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVPENVSPGQAFWAPGDAGVVF
Gene ID
Swiss Prot
SynonymsAPH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2
Calculated MW81kDa
Observed MW80-108kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSW480, Jurkat, Mouse liver, Mouse kidney, Mouse small intestine, Rat bone marrow
Cellular locationCytoplasm

Documents

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ABclonal:Western blot - APEH Rabbit pAb (A13467)}

Western blot - APEH Rabbit pAb (A13467)

Western blot analysis of extracts of various cell lines, using APEH antibody (A13467) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - APEH Rabbit pAb (A13467)}

Immunofluorescence - APEH Rabbit pAb (A13467)

Immunofluorescence analysis of HeLa cells using APEH antibody (A13467). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)