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[KO Validated] APEH Rabbit pAb (A5893)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - [KO Validated] APEH Rabbit pAb (A5893)

Western blot analysis of various lysates, using [KO Validated] APEH Rabbit pAb (A5893) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - [KO Validated] APEH Rabbit pAb (A5893)

Western blot analysis of lysates from wild type(WT) and APEH knockout (KO) 293T(KO) cells, using [KO Validated] APEH Rabbit pAb (A5893) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunoprecipitation - [KO Validated] APEH Rabbit pAb (A5893)

Immunoprecipitation analysis of 100 μg extracts of SW480 cells using 3 μg APEH antibody (A5893). Western blot was performed from the immunoprecipitate using APEH antibody (A5893) at a dilution of 1:1000.

Overview

Product name[KO Validated] APEH Rabbit pAb
Catalog No.A5893
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3).
SequenceMERQVLLSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVLRKAGGTGPGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVPENVSPGQAFWAPGDAGVVF
Gene ID
Swiss Prot
SynonymsAPH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2; EH
Calculated MW81kDa
Observed MW81kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunoprecipitation    
Positive samples293T, Jurkat
Cellular locationCytoplasm

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - [KO Validated] APEH Rabbit pAb (A5893)}

Western blot - [KO Validated] APEH Rabbit pAb (A5893)

Western blot analysis of various lysates, using [KO Validated] APEH Rabbit pAb (A5893) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - [KO Validated] APEH Rabbit pAb (A5893)}

Western blot - [KO Validated] APEH Rabbit pAb (A5893)

Western blot analysis of lysates from wild type(WT) and APEH knockout (KO) 293T(KO) cells, using [KO Validated] APEH Rabbit pAb (A5893) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunoprecipitation - [KO Validated] APEH Rabbit pAb (A5893)}

Immunoprecipitation - [KO Validated] APEH Rabbit pAb (A5893)

Immunoprecipitation analysis of 100 μg extracts of SW480 cells using 3 μg APEH antibody (A5893). Western blot was performed from the immunoprecipitate using APEH antibody (A5893) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)