Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human
Western blot analysis of lysates from PC-3 cells, using ARRDC4 pAb (A18522) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
| Product name | ARRDC4 Rabbit pAb |
|---|---|
| Catalog No. | A18522 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human ARRDC4 (NP_899232.2). |
|---|---|
| Sequence | PGAKKLMLELPLVIGTIPYNGFGSRNSSIASQFSMDMSWLTLTLPEQPEAPPNYADVVSEEEFSRHIPPYPQPPNCEGEVCCPVFACIQEFRFQPPPLYSE |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ARRDC4 |
| Calculated MW | 45kDa |
| Observed MW | 45kDa |
| Reactivity | Human |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | PC-3 |
| Cellular location | cytoplasm, early endosome, endosome, extracellular vesicle, plasma membrane |
| Customer validation | IHC(Mus musculus) |
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A18522? Please let us know so that we can cite the reference in this datasheet.
