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ASAH2 Rabbit pAb (A12596)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ASAH2 Rabbit pAb (A12596)

Western blot analysis of extracts of various cell lines, using ASAH2 antibody (A12596) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameASAH2 Rabbit pAb
Catalog No.A12596
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human ASAH2 (NP_001137446.1).
SequenceTATQTSPVPLTPESPLFQNFSGYHIGVGRADCTGQVADINLMGYGKSGQNAQGILTRLYSRAFIMAEPDGSNRTVFVSIDIGMVSQRLRLEVLNRLQSKYGSLYRRDNVILSGTHTHSGPAGYFQYTVFVIASEGFSNQTFQHMVTGILKSIDIAHTNMKPGKIFINKGNVDGVQINRSPYSYLQNPQSERARYSSNTDKEMIVLKMVDLNGDDLGLISWFAIHPVSMNNSNHLVNSDNVGYASYLLEQEKNKGYLPGQGP
Gene ID
Swiss Prot
SynonymsHNAC1; BCDase; LCDase; NCDase; N-CDase
Calculated MW86kDa
Observed MW85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples22Rv1, Mouse kidney, Rat kidney
Cellular locationCell membrane, Single-pass type II membrane protein
Customer validation

WB(Homo sapiens)

Documents

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    ABclonal:Western blot - ASAH2 Rabbit pAb (A12596)}

    Western blot - ASAH2 Rabbit pAb (A12596)

    Western blot analysis of extracts of various cell lines, using ASAH2 antibody (A12596) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A12596? Please let us know so that we can cite the reference in this datasheet.

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    Secondary Antibodies (26)