제품 > 항체 > 다중클론항체(pAb)

ATAD3A/ATAD3B Rabbit pAb (A22703)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various lysates, using ATAD3A/ATAD3B antibody (A22703) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ATAD3A/ATAD3B Rabbit pAb (A22703)

Immunofluorescence analysis of U20S cells using ATAD3A/ATAD3B antibody (A22703) at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using ATAD3A/ATAD3B antibody (A22703) at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

Immunofluorescence analysis of H9C2 cells using ATAD3A/ATAD3B antibody (A22703) at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining.

Overview

Product name	ATAD3A/ATAD3B Rabbit pAb
Catalog No.	A22703
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is localized to the mitochondrial inner membrane, where it can bind to a highly-related protein, ATAD3A. ATAD3A appears to interact with matrix nucleoid complexes, and the encoded protein negatively regulates that interaction. This gene is expressed almost exclusively in pluripotent embryonic stem cells and some cancer cells. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3B (NP_114127.3).
Sequence	MSWLFGVNKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKEALNLAQMQEQTLQLEQQSKLKEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQQLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVETEARARAKAERENADIIREQIRLKASEHRQTVLESIRTAGTLFGEGFRAF
Gene ID
Swiss Prot
Synonyms	TOB3; AAA-TOB3
Calculated MW	71/73kDa
Observed MW	68kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, A-549, U-251MG
Cellular location	Mitochondrion inner membrane, Peripheral membrane protein