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ATAD3A/B Rabbit mAb (A23184)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATAD3A/B Rabbit mAb (A23184)

Western blot analysis of various lysates, using ATAD3A/B Rabbit mAb (A23184) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameATAD3A/B Rabbit mAb
Catalog No.A23184
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC59679
This gene encodes a ubiquitously expressed mitochondrial membrane protein that contributes to mitochondrial dynamics, nucleoid organization, protein translation, cell growth, and cholesterol metabolism. This gene is a member of the ATPase family AAA-domain containing 3 gene family which, in humans, includes two other paralogs. Naturally occurring mutations in this gene are associated with distinct neurological syndromes including Harel-Yoon syndrome. High-level expression of this gene is associated with poor survival in breast cancer patients. A homozygous knockout of the orthologous gene in mice results in embryonic lethality at day 7.5 due to growth retardation and defective development of the trophoblast lineage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human ATAD3A/B. (NP_060658.3).
SequenceMSWLFGINKGPKGEGAGPPPPLPPAQPGAEGGGDRGLGDRPAPKDKWSNFDPTGLERAAKAARELEHSRYAKDALNLAQMQEQTLQLEQQSKLKMRLEALSLLHTLVWAWSLCRAGAVQTQERLSGSASPEQVPAGECCALQEYEAAVEQLKSEQIRAQAEERRKTLSEETRQHQARAQYQDKLARQRYEDQLKQQQLLNEENLRKQEESVQKQEAMRRATVEREMELRHKNEMLRVEAE
Gene ID
Swiss Prot
SynonymsATAD3A; HAYOS; ATPase family; ATAD3A/B
Calculated MW57kDa/66kDa/71kDa
Observed MW71kDa、72.5kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesU-937, HeLa, 293F, Mouse liver
Cellular locationMitochondrion inner membrane, Mitochondrion matrix, Single-pass membrane protein, mitochondrion nucleoid

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ATAD3A/B Rabbit mAb (A23184)}

    Western blot - ATAD3A/B Rabbit mAb (A23184)

    Western blot analysis of various lysates, using ATAD3A/B Rabbit mAb (A23184) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)}

    Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

    Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)}

    Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)}

    Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)}

    Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

    Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)}

    Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

    Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)}

    Immunohistochemistry - ATAD3A/B Rabbit mAb (A23184)

    Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ATAD3A/B Rabbit mAb (A23184) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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