ATF6 Rabbit mAb (A25317)

Western blot analysis of lysates from HeLa cells using ATF6 Rabbit mAb (A25317) at 1:5000 dilution incubated overnight at 4°C. HeLa cells were treated by tunicamycin (2 μg/ml) for 1 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 90 s.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ATF6 Rabbit mAb (A25317) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ATF6 Rabbit mAb (A25317) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunoprecipitation of ATF6 from 500 µg extracts of HeLa cells was performed using 3 µg of ATF6 Rabbit mAb (A25317). Rabbit IgG isotype control(AC042) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using ATF6 Rabbit mAb (A25317) at a dilution of 1:1000.

Chromatin immunoprecipitation was performed with 20 μg of cross-linked chromatin from HeLa cells (left) and HeLa cells treated by thapsigargin (1uM, 1h)(right), using 2 μg of ATF6 Rabbit mAb (A25317) and Rabbit IgG isotype control (AC042). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.