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ATG4A Rabbit pAb (A2598)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATG4A Rabbit pAb (A2598)

Western blot analysis of extracts of various cell lines, using ATG4A antibody (A2598) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - ATG4A Rabbit pAb (A2598)

Immunofluorescence analysis of NIH/3T3 cells using ATG4A Rabbit pAb (A2598) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameATG4A Rabbit pAb
Catalog No.A2598
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 210-398 of human ATG4A (NP_443168.2).
SequenceSKGTSAYCSAWKPLLLIVPLRLGINQINPVYVDAFKECFKMPQSLGALGGKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEENGTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDNWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEEDFEILSV
Gene ID
Swiss Prot
SynonymsAPG4A; AUTL2; HsAPG4A
Calculated MW45kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, Mouse liver, Rat lung
Cellular locationCytoplasm

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ABclonal:Western blot - ATG4A Rabbit pAb (A2598)}

Western blot - ATG4A Rabbit pAb (A2598)

Western blot analysis of extracts of various cell lines, using ATG4A antibody (A2598) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - ATG4A Rabbit pAb (A2598)}

Immunofluorescence - ATG4A Rabbit pAb (A2598)

Immunofluorescence analysis of NIH/3T3 cells using ATG4A Rabbit pAb (A2598) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)