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[KO Validated] ATG4A Rabbit pAb (A21356)

KO/KDValidated

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)

Western blot analysis of various lysates using [KO Validated] ATG4A Rabbit pAb (A21356) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)

Western blot analysis of lysates from wild type (WT) and ATG4A knockout (KO) HeLa cells using [KO Validated] ATG4A Rabbit pAb (A21356) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)

Western blot analysis of lysates from HeLa cells using [KO Validated] ATG4A Rabbit pAb (A21356) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name[KO Validated] ATG4A Rabbit pAb
Catalog No.A21356
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 289-398 of human ATG4A (NP_443168.2).
SequenceTEENGTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKEEKDFDNWCSLVQKEILKENLRMFELVQKHPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEEDFEILSV
Gene ID
Swiss Prot
SynonymsAPG4A; AUTL2; HsAPG4A; ATG4A
Calculated MW45kDa
Observed MW45kDa/50kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, K-562, HeLa, Jurkat, HeLa
Cellular locationCytoplasm.

Documents

Certificate of Compliance

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ABclonal:Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)}

Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)

Western blot analysis of various lysates using [KO Validated] ATG4A Rabbit pAb (A21356) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)}

Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)

Western blot analysis of lysates from wild type (WT) and ATG4A knockout (KO) HeLa cells using [KO Validated] ATG4A Rabbit pAb (A21356) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)}

Western blot - [KO Validated] ATG4A Rabbit pAb (A21356)

Western blot analysis of lysates from HeLa cells using [KO Validated] ATG4A Rabbit pAb (A21356) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

* For research use only. Not for therapeutic or diagnostic purposes.

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