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ATP1B3 Rabbit mAb (A19637)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP1B3 Rabbit mAb (A19637)

Western blot analysis of various lysates using ATP1B3 Rabbit mAb (A19637) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - ATP1B3 Rabbit mAb (A19637)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ATP1B3 Rabbit mAb (A19637) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameATP1B3 Rabbit mAb
Catalog No.A19637
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2205
The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 180-279 of human ATP1B3 (P54709).
SequenceGLKPEGVPRIDCVSKNEDIPNVAVYPHNGMIDLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKEVTVECKIDGSANLKSQDDRDKFLGRVMFKITARA
Gene ID
Swiss Prot
SynonymsCD298; ATPB-3; ATP1B3
Calculated MW22kDa/32kDa
Observed MW32-45kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, U-87MG, Mouse lung, Mouse testis, Mouse brain, Rat lung, Rat testis, Rat spleen
Cellular locationapical plasma membrane, basolateral plasma membrane, extracellular exosome, melanosome, plasma membrane, sperm flagellum.

Documents

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ABclonal:Western blot - ATP1B3 Rabbit mAb (A19637)}

Western blot - ATP1B3 Rabbit mAb (A19637)

Western blot analysis of various lysates using ATP1B3 Rabbit mAb (A19637) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - ATP1B3 Rabbit mAb (A19637)}

Immunohistochemistry - ATP1B3 Rabbit mAb (A19637)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ATP1B3 Rabbit mAb (A19637) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)