Western blot - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Western blot analysis of various lysates using CD298/ATP1B3 Rabbit PolymAb® (A19637PM) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Immunohistochemistry analysis of paraffin-embedded Human liver tissue using CD298/ATP1B3 Rabbit PolymAb® (A19637PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using CD298/ATP1B3 Rabbit PolymAb® (A19637PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using CD298/ATP1B3 Rabbit PolymAb® (A19637PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas tissue using CD298/ATP1B3 Rabbit PolymAb® (A19637PM) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunofluorescence - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Confocal imaging of 293T cells using CD298/ATP1B3 Rabbit PolymAb® (A19637PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Confocal imaging of Jurkat cells using CD298/ATP1B3 Rabbit PolymAb® (A19637PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Immunofluorescence - CD298/ATP1B3 Rabbit PolymAb® (A19637PM)
Confocal imaging of paraffin-embedded Human liver tissue using CD298/ATP1B3 Rabbit PolymAb® (A19637PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.