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ATP2B1 Rabbit mAb (A24346)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATP2B1 Rabbit mAb (A24346)

Western blot analysis of various lysates, using ATP2B1 Rabbit mAb (A24346) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - ATP2B1 Rabbit mAb (A24346)

Immunofluorescence analysis of HeLa cells using ATP2B1 Rabbit mAb (A24346) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATP2B1 Rabbit mAb (A24346)

Immunofluorescence analysis of NIH/3T3 cells using ATP2B1 Rabbit mAb (A24346) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATP2B1 Rabbit mAb (A24346)

Immunofluorescence analysis of PC-12 cells using ATP2B1 Rabbit mAb (A24346) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameATP2B1 Rabbit mAb
Catalog No.A24346
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61750
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 5-103 of human ATP2B1 (NP_001673.2).
SequenceANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNFIPPKKPKTFLQLVW
Gene ID
Swiss Prot
SynonymsMRD66; PMCA1; PMCA1kb;
Calculated MW135kDa
Observed MW135-180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesTHP-1(Low expression control), A549, Neuro-2a, Rat lung
Cellular locationCell membrane

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ABclonal:Western blot - ATP2B1 Rabbit mAb (A24346)}

Western blot - ATP2B1 Rabbit mAb (A24346)

Western blot analysis of various lysates, using ATP2B1 Rabbit mAb (A24346) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - ATP2B1 Rabbit mAb (A24346)}

Immunofluorescence - ATP2B1 Rabbit mAb (A24346)

Immunofluorescence analysis of HeLa cells using ATP2B1 Rabbit mAb (A24346) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATP2B1 Rabbit mAb (A24346)}

Immunofluorescence - ATP2B1 Rabbit mAb (A24346)

Immunofluorescence analysis of NIH/3T3 cells using ATP2B1 Rabbit mAb (A24346) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATP2B1 Rabbit mAb (A24346)}

Immunofluorescence - ATP2B1 Rabbit mAb (A24346)

Immunofluorescence analysis of PC-12 cells using ATP2B1 Rabbit mAb (A24346) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)