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Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738)

Immunohistochemistry analysis of Acetyl-alpha Tubulin -K40 in paraffin-embedded human brain, using Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Acetyl-alpha Tubulin -K40 in paraffin-embedded mouse kidney, using Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738)

Confocal imaging of Hep G2 cells using Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Confocal imaging of 293T cells using Acetyl-alpha Tubulin -K40 Rabbit mAb (A24738, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

Review

Overview

Product name	Acetyl-alpha Tubulin -K40 Rabbit mAb
Catalog No.	A24738
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC64227

Background

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blot studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, intellectual disability, and early-onset epilepsy caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen information

Immunogen	A synthetic acetylated peptide around K40 of human alpha Tubulin(NP_001257328.1).
Sequence	C-DGQNlePSDK(Ac)TIGG
Gene ID
Swiss Prot
Synonyms	LIS3; TUBA3; B-ALPHA-1; Acetyl-alpha Tubulin -K40
Calculated MW	50kDa
Observed MW	55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse testis, Rat testis
Cellular location	Cytoplasm, cytoskeleton
Customer validation	(fibroblast) WB(Homo sapiens)