제품 > 항체 > 단일클론항체(mAb)

Alcohol Dehydrogenase Rabbit mAb (A19624)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse,

Western blot analysis of lysates from Mouse lung, using Alcohol Dehydrogenase Rabbit mAb (A19624) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Alcohol Dehydrogenase Rabbit mAb
Catalog No.	A19624
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2203

Background

This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence. [provided by RefSeq, Nov 2010]

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alcohol Dehydrogenase (P07327).
Sequence	MSTAGKVIKCKAAVLWELKKPFSIEEVEVAPPKAHEVRIKMVAVGICGTDDHVVSGTMVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLAIPQCGK
Gene ID
Swiss Prot
Synonyms	ADH1; Alcohol Dehydrogenase
Calculated MW	40kDa
Observed MW	40kDa

Applications

Reactivity	Human, Mouse,
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	LO2, Mouse liver, Mouse lung
Cellular location	Cytosol, Nucleoplasm, Plasma membrane
Customer validation	WB(Mus musculus)