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Alcohol Dehydrogenase Rabbit pAb (A14701)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - Alcohol Dehydrogenase Rabbit pAb (A14701)

Western blot analysis of various lysates using Alcohol Dehydrogenase Rabbit pAb (A14701) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameAlcohol Dehydrogenase Rabbit pAb
Catalog No.A14701
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the alcohol dehydrogenase family. The encoded protein is the alpha subunit of class I alcohol dehydrogenase, which consists of several homo- and heterodimers of alpha, beta and gamma subunits. Alcohol dehydrogenases catalyze the oxidation of alcohols to aldehydes. This gene is active in the liver in early fetal life but only weakly active in adult liver. This gene is found in a cluster with six additional alcohol dehydrogenase genes, including those encoding the beta and gamma subunits, on the long arm of chromosome 4. Mutations in this gene may contribute to variation in certain personality traits and substance dependence.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 220-300 of human Alcohol Dehydrogenase (NP_000658.1).
SequenceIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPDSQ
Gene ID
Swiss Prot
SynonymsADH1A; ADH1; Alcohol Dehydrogenase
Calculated MW40kDa
Observed MW40kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesLO2, Rat liver
Cellular locationCytoplasm
    ABclonal:Western blot - Alcohol Dehydrogenase Rabbit pAb (A14701)}

    Western blot - Alcohol Dehydrogenase Rabbit pAb (A14701)

    Western blot analysis of various lysates using Alcohol Dehydrogenase Rabbit pAb (A14701) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)