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Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of mouse brain, using Alpha-tubulin (ubiquitous) chain antibody (AC003).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Overview

Product name	Alpha-tubulin (ubiquitous) chain Rabbit pAb
Catalog No.	AC003
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables double-stranded RNA binding activity and ubiquitin protein ligase binding activity. Predicted to be involved in microtubule cytoskeleton organization and mitotic cell cycle. Predicted to act upstream of or within cellular response to interleukin-4. Located in microtubule.

Immunogen information

Immunogen	Recombinant protein of human Alpha-tubulin (ubiquitous) chain
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	K-ALPHA-1
Calculated MW	50kDa
Observed MW	50kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:20 - 1:100 IP 1:20 - 1:50 FC 1:20 - 1:50
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation Flow Cytometry
Positive samples	Mouse brain
Cellular location	Cytoplasm, cytoskeleton
Customer validation	WB(Mus musculus, Homo sapiens, Rattus norvegicus) IP(Homo sapiens)

Research Area

Alpha-tubulin (ubiquitous) chain Rabbit pAb images

Western blot - Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Biology Of Reproduction

Application:

IF:

3.1

Species:

Mus musculus

PMID:

27605345

Title:

mTORC1 Activation Promotes Spermatogonial Differentiation and Causes Subfertility in Mice

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

BMC cancer

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

32041570

Title:

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Psychopharmacology

Application:

IF:

3.5

Species:

Mus musculus

PMID:

31953648

Title:

Vortioxetine administration attenuates cognitive and synaptic deficits in 5×FAD mice

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Toxicology and Applied Pharmacology

Application:

IF:

3.3

Species:

Rattus norvegicus

PMID:

32234517

Title:

miR-148a/LDLR mediates hypercholesterolemia induced by prenatal dexamethasone exposure in male offspring rats

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

BMC Cancer

Application:

IF:

3.8

Species:

Homo sapiens

PMID:

32041570

Title:

Linc01234 promotes cell proliferation and metastasis in oral squamous cell carcinoma via miR-433/PAK4 axis.

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Biochem Pharmacol

Application:

IF:

5.3

Species:

Mus musculus

PMID:

32615080

Title:

GR-C/EBPα-IGF1 axis mediated azithromycin-induced liver developmental toxicity in fetal mice

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Biochemical pharmacology

Application:

IF:

5.3

Species:

Rattus norvegicus

PMID:

36326533

Title:

MiR-133a-3p/Sirt1 epigenetic programming mediates hypercholesterolemia susceptibility in female offspring induced by prenatal dexamethasone exposure

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Biomedicines

Application:

IF:

4.7

Species:

Mus musculus

PMID:

36359279

Title:

Isoflurane Rescue Schizophrenia-Related Deficits through Parvalbumin-Positive Neurons in the Dentate Gyrus - PMC

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Frontiers in cellular neuroscience

Application:

IF:

6.1

Species:

Mus musculus

PMID:

38026699

Title:

Parvalbumin-positive neurons in the medial vestibular nucleus contribute to vestibular compensation through commissural inhibition - PMC

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Rattus norvegicus

PMID:

37622292

Title:

mGluR1/IP3/ERK signaling pathway regulates vestibular compensation in ON UBCs of the cerebellar flocculus

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Cell Cycle

Application:

WB, IP

IF:

3.4

Species:

Homo sapiens

PMID:

27341063

Title:

EWSR1 regulates mitosis by dynamically influencing microtubule acetylation.

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Molecular neurobiology

Application:

IF:

4.6

Species:

Mus musculus

PMID:

38008888

Title:

Uric Acid Mitigates Cognitive Deficits via TFEB-Mediated Microglial Autophagy in Mice Models of Alzheimer's Disease

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Neoplasia (New York, N.Y.)

Application:

IF:

6.3

Species:

Mus musculus

PMID:

38237535

Title:

AEBP1 promotes papillary thyroid cancer progression by activating BMP4 signaling - PMC

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Food & function

Application:

IF:

5.1

Species:

Rattus norvegicus

PMID:

38113052

Title:

The high-expression programming of SR-B1 mediates adrenal dysfunction in female offspring induced by prenatal caffeine exposure and its cholesterol accumulation

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Cellular and molecular neurobiology

Application:

IF:

3.6

Species:

Mus musculus

PMID:

38285192

Title:

Treadmill running regulates adult neurogenesis, spatial and non-spatial learning, parvalbumin neuron activity by ErbB4 signaling

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Journal of ethnopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

38278375

Title:

Cornus officinalis var. koreana Kitam extracts alleviate cadmium-induced renal fibrosis by targeting matrix metallopeptidase 9

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

Frontiers in Cell and Developmental Biology

Application:

IF:

4.6

Species:

Mus musculus

PMID:

32117963

Title:

Parvalbumin Interneuron Activation-Dependent Adult Hippocampal Neurogenesis Is Required for Treadmill Running to Reverse Schizophrenia-Like Phenotypes

References for Alpha-tubulin (ubiquitous) chain Rabbit pAb (AC003)

Product:

Alpha-tubulin (ubiquitous) chain Rabbit pAb

Journal:

CNS neuroscience & therapeutics

Application:

IF:

5.5

Species:

Mus musculus

PMID:

37248637