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Apolipoprotein A2 Rabbit pAb (A14690)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Apolipoprotein A2 Rabbit pAb (A14690)

Western blot analysis of lysates from human plasma, using Apolipoprotein A2 Rabbit pAb (A14690) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Apolipoprotein A2 Rabbit pAb (A14690)

Immunofluorescence analysis of C6 cells using Apolipoprotein A2 Rabbit pAb (A14690) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Apolipoprotein A2 Rabbit pAb (A14690)

Immunofluorescence analysis of NIH/3T3 cells using Apolipoprotein A2 Rabbit pAb (A14690) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameApolipoprotein A2 Rabbit pAb
Catalog No.A14690
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Apolipoprotein A2 (NP_001634.1).
SequenceMKLLAATVLLLTICSLEGALVRRQAKEPCVESLVSQYFQTVTDYGKDLMEKVKSPELQAEAKSYFEKSKEQLTPLIKKAGTELVNFLSYFVELGTQPATQ
Gene ID
Swiss Prot
SynonymsapoAII; Apo-AII; ApoA-II; Apolipoprotein A2
Calculated MW17kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHuman plasma
Cellular locationSecreted

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ABclonal:Western blot - Apolipoprotein A2 Rabbit pAb (A14690)}

Western blot - Apolipoprotein A2 Rabbit pAb (A14690)

Western blot analysis of lysates from human plasma, using Apolipoprotein A2 Rabbit pAb (A14690) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - Apolipoprotein A2 Rabbit pAb (A14690)}

Immunofluorescence - Apolipoprotein A2 Rabbit pAb (A14690)

Immunofluorescence analysis of C6 cells using Apolipoprotein A2 Rabbit pAb (A14690) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Apolipoprotein A2 Rabbit pAb (A14690)}

Immunofluorescence - Apolipoprotein A2 Rabbit pAb (A14690)

Immunofluorescence analysis of NIH/3T3 cells using Apolipoprotein A2 Rabbit pAb (A14690) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (25)