제품 > 항체 > 다중클론항체(pAb)

Arginase-1 Rabbit pAb (A23648)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of lysates from Mouse liver using Arginase-1 Rabbit pAb(A23648) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from Rat liver using Arginase-1 Rabbit pAb(A23648) at 1:5000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunofluorescence - Arginase-1 Rabbit pAb (A23648)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded mouse liver using Arginase-1 Rabbit pAb (A23648) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Arginase-1 Rabbit pAb
Catalog No.	A23648
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables arginase activity. Involved in defense response to protozoan; negative regulation of T-helper 2 cell cytokine production; and negative regulation of activated T cell proliferation. Predicted to be located in several cellular components, including extracellular space; mitochondrial outer membrane; and neuronal cell body. Predicted to be active in cytosol. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; integumental system; and sensory organ. Used to study hyperargininemia. Human ortholog(s) of this gene implicated in asthma; hepatocellular carcinoma; and hyperargininemia. Orthologous to human ARG1 (arginase 1).

Immunogen information

Immunogen	Recombinant protein of mouse Arginase-1.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	AI; PGIF; Arg-1; Arginase-1
Calculated MW	34KDa
Observed MW	40kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse liver, Rat liver
Cellular location
Customer validation	IHC(Mus musculus) IF(Mus musculus)