제품 > 항체 > 다중클론항체(pAb)

BAG1 Rabbit pAb (A1104)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - BAG1 Rabbit pAb (A1104)

Western blot analysis of extracts of various cell lines, using BAG1 antibody (A1104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - BAG1 Rabbit pAb (A1104)

Immunofluorescence analysis of U2OS cells using BAG1 antibody (A1104). Blue: DAPI for nuclear staining.

Overview

Product nameBAG1 Rabbit pAb
Catalog No.A1104
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BAG1 (NP_001165886.1).
SequenceMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEMETPLSALGIQDGCRVMLIGKKNSPQEEVELKKLKHLEKSVEKIADQLEELNKELTGIQQGFLPKDLQAEALCKLDRRVKATIEQFMKILEEIDTLILPENFKDSRLKRKGLVKKVQAFLAECDTVEQNICQETERLQSTNFALAE
Gene ID
Swiss Prot
SynonymsHAP; BAG-1; RAP46
Calculated MW39kDa
Observed MW43kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesSW620, Mouse kidney
Cellular locationCytoplasm, Cytoplasm, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - BAG1 Rabbit pAb (A1104)}

Western blot - BAG1 Rabbit pAb (A1104)

Western blot analysis of extracts of various cell lines, using BAG1 antibody (A1104) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunofluorescence - BAG1 Rabbit pAb (A1104)}

Immunofluorescence - BAG1 Rabbit pAb (A1104)

Immunofluorescence analysis of U2OS cells using BAG1 antibody (A1104). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)