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Bag1 Rabbit mAb (A20863)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Bag1 Rabbit mAb (A20863)

Western blot analysis of various lysates using Bag1 Rabbit mAb (A20863) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Bag1 Rabbit mAb (A20863)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Bag1 Rabbit mAb (A20863) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Bag1 Rabbit mAb (A20863)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Bag1 Rabbit mAb (A20863) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Bag1 Rabbit mAb (A20863)

Confocal imaging of NIH/3T3 cells using Bag1 Rabbit mAb (A20863, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - Bag1 Rabbit mAb (A20863)

Confocal imaging of PC-12 cells using Bag1 Rabbit mAb (A20863, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameBag1 Rabbit mAb
Catalog No.A20863
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2791
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human Bag1 (Q99933).
SequenceWSEEATQSEEATQGEEMNRSQEVTRDEESTRSEEVTREEMAAAGLTVTVTHSNEKHDLHVTSQQGSSEPVVQDLAQVVEEVIGVPQSFQKLIFKGKSLKEM
Gene ID
Swiss Prot
SynonymsHAP; BAG-1; RAP46; Bag1
Calculated MW39kDa
Observed MW33kDa/46kDa/52kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Jurkat, Mouse lung, Mouse testis, Rat testis
Cellular locationCytoplasm, Cytoplasm, Nucleus.
Customer validation

RIP(Drosophila melanogaster)

Documents

Certificate of Compliance

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ABclonal:Western blot - Bag1 Rabbit mAb (A20863)}

Western blot - Bag1 Rabbit mAb (A20863)

Western blot analysis of various lysates using Bag1 Rabbit mAb (A20863) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Bag1 Rabbit mAb (A20863)}

Immunohistochemistry - Bag1 Rabbit mAb (A20863)

Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Bag1 Rabbit mAb (A20863) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Bag1 Rabbit mAb (A20863)}

Immunohistochemistry - Bag1 Rabbit mAb (A20863)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Bag1 Rabbit mAb (A20863) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Bag1 Rabbit mAb (A20863)}

Immunofluorescence - Bag1 Rabbit mAb (A20863)

Confocal imaging of NIH/3T3 cells using Bag1 Rabbit mAb (A20863, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - Bag1 Rabbit mAb (A20863)}

Immunofluorescence - Bag1 Rabbit mAb (A20863)

Confocal imaging of PC-12 cells using Bag1 Rabbit mAb (A20863, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)