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BCL2L14/BCLG Rabbit pAb (A6478)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BCL2L14/BCLG Rabbit pAb (A6478)

Western blot analysis of extracts of various cell lines, using BCL2L14/BCLG antibody (A6478) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - BCL2L14/BCLG Rabbit pAb (A6478)

Immunofluorescence analysis of HeLa cells using BCL2L14/BCLG antibody (A6478). Blue: DAPI for nuclear staining.

Overview

Product nameBCL2L14/BCLG Rabbit pAb
Catalog No.A6478
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of this gene has been shown to induce apoptosis in cells. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human BCL2L14/BCLG (NP_620049.1).
SequenceMCSTSGCDLEEIPLDDDDLNTIEFKILAYYTRHHVFKSTPALFSPKLLRTRSLSQRGLGNCSANESWTEVSWPCRNSQSSEKAINLGKKKSSWKAFFGVVEKEDSQSTPAKVSAQGQRTLEYQDSHSQQWSRCLSNVEQCLEHEAVDPKVISIANRVAEIVYSWPPPQATQAGGFKSKEIFVTEGLSFQLQGHVPVASSSKKDEEEQILAKIVELLKYSGDQLERKLKKDKALMGHFQDGLSYSVFKTITDQVLMGVDPRGESEVKAQGFKAALVIDVTAKLTAIDNHPMNRVLGFGTKYLKENFSPWIQQHGGWEKILGISHEEVD
Gene ID
Swiss Prot
SynonymsBCLG
Calculated MW37kDa
Observed MW39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:10 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse liver, Rat liver, Rat brain
Cellular locationCytoplasm, Endomembrane system, cytosol

Documents

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    ABclonal:Western blot - BCL2L14/BCLG Rabbit pAb (A6478)}

    Western blot - BCL2L14/BCLG Rabbit pAb (A6478)

    Western blot analysis of extracts of various cell lines, using BCL2L14/BCLG antibody (A6478) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Immunofluorescence - BCL2L14/BCLG Rabbit pAb (A6478)}

    Immunofluorescence - BCL2L14/BCLG Rabbit pAb (A6478)

    Immunofluorescence analysis of HeLa cells using BCL2L14/BCLG antibody (A6478). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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