제품 > 항체 > 다중클론항체(pAb)

BG1 Rabbit pAb (A20703)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - BG1 Rabbit pAb (A20703)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using BG1 Rabbit pAb (A20703) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameBG1 Rabbit pAb
Catalog No.A20703
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in auxin transport. Positive regulator of the auxin signaling pathway involved in gravitropism, plant growth and grain development.
ImmunogenRecombinant protein of Oryza sativa BG1.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsBG1; OsJ_09620
Calculated MW33kDa
Observed MW37kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmilk grain
Cellular locationplasma membrane
    ABclonal:Western blot - BG1 Rabbit pAb (A20703)}

    Western blot - BG1 Rabbit pAb (A20703)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using BG1 Rabbit pAb (A20703) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)