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BRAF V600E Rabbit mAb (A24020)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - BRAF V600E Rabbit mAb (A24020)

Western blot analysis of lysates from wild type (WT) , 293T cells transfected with BRAF and 293T cells transfected with BRAF V600E using B-Raf Rabbit mAb (A24020) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.

ABclonal:Immunofluorescence - BRAF V600E Rabbit mAb (A24020)

Confocal imaging of 293F-BRAF V600E and 293F-BRAF and 293F cells using BRAF V600E Rabbit mAb (A24020, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameBRAF V600E Rabbit mAb
Catalog No.A24020
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62593
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 507-606 of B-Raf (NP_004324.2).
SequenceKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSG
Gene ID
Swiss Prot
SynonymsNS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; BRAF V600E
Calculated MW84kDa
Observed MW85kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IF 1:200- 1 : 800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T transfected with BRAF V600E
Cellular locationCell membrane, Cytoplasm, Nucleus.
Customer validation

WB(Sus scrofa)

Documents

Certificate of Compliance

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ABclonal:Western blot - BRAF V600E Rabbit mAb (A24020)}

Western blot - BRAF V600E Rabbit mAb (A24020)

Western blot analysis of lysates from wild type (WT) , 293T cells transfected with BRAF and 293T cells transfected with BRAF V600E using B-Raf Rabbit mAb (A24020) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.
ABclonal:Immunofluorescence - BRAF V600E Rabbit mAb (A24020)}

Immunofluorescence - BRAF V600E Rabbit mAb (A24020)

Confocal imaging of 293F-BRAF V600E and 293F-BRAF and 293F cells using BRAF V600E Rabbit mAb (A24020, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

Secondary Antibodies (26)