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Phospho-B-Raf-T401 Rabbit mAb (AP1041)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rat

ABclonal:Western blot - Phospho-B-Raf-T401 Rabbit mAb (AP1041)

Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (AP1041) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product namePhospho-B-Raf-T401 Rabbit mAb
Catalog No.AP1041
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1587
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
ImmunogenA synthetic phosphorylated peptide around T401 of human-B Raf (NP_004324.2).
SequenceSATPP
Gene ID
Swiss Prot
SynonymsNS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; Phospho-B-Raf-T401
Calculated MW84kDa
Observed MW85kDa
ReactivityRat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesC6 treated by PMA
Cellular locationCell membrane, Cytoplasm, Nucleus.

Documents

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ABclonal:Western blot - Phospho-B-Raf-T401 Rabbit mAb (AP1041)}

Western blot - Phospho-B-Raf-T401 Rabbit mAb (AP1041)

Western blot analysis of lysates from C6 cells, using Phospho-B-Raf-T401 Rabbit mAb (AP1041) at 1:1000 dilution. C6 cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

* For research use only. Not for therapeutic or diagnostic purposes.

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