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B-Raf V600E Rabbit pAb (A25185)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot -  B-Raf V600E Rabbit pAb (A25185)

Western blot analysis of lysates from wild type (WT) , 293T cells transfected with BRAF and 293T cells transfected with BRAF V600E using B-Raf Rabbit pAb (A25185) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name B-Raf V600E Rabbit pAb
Catalog No.A25185
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 507-606 of B-Raf V600E (NP_004324.2).
SequenceKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATEKSRWSG
Gene ID
Swiss Prot
SynonymsNS7; B-raf; BRAF1; RAFB1; B-RAF1; BRAF-1; B-Raf
Calculated MW84kDa
Observed MW85kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293F transfected with BRAF V600E
Cellular locationCell membrane, Cytoplasm, Nucleus

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ABclonal:Western blot -  B-Raf V600E Rabbit pAb (A25185)}

Western blot - B-Raf V600E Rabbit pAb (A25185)

Western blot analysis of lysates from wild type (WT) , 293T cells transfected with BRAF and 293T cells transfected with BRAF V600E using B-Raf Rabbit pAb (A25185) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (6)

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