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BRRF2 Rabbit mAb (A23835)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Epstein-Barr virus

ABclonal:Western blot - BRRF2 Rabbit mAb (A23835)

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with BRRF2 using BRRF2 Rabbit mAb (A23835) at 1:2500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunofluorescence - BRRF2 Rabbit mAb (A23835)

Immunofluorescence analysis of 293F-BRRF2 and 293F cells using BRRF2 Rabbit mAb (A23835) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBRRF2 Rabbit mAb
Catalog No.A23835
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC61624
BRRF2 is a capsid protein, but it can be secreted into the bloodstream, giving it the opportunity to be a diagnostic or immunotherapy target.The Epstein–Barr virus BRRF2 gene product is involved in viral progeny production .
ImmunogenRecombinant protein of epstein-Barr virus (strain B95-8) (HHV-4) BRRF2.
SequenceMSGQQRGSVILVPEHLAGALTKLMSDFITGQDVTLSGGNIAVKIRDAINQTPGGGDVAILSSLFALWNALPTSGRQSSRDDLIPAAVQALTTAHNLCLGVIPGETSHKDTPESLLRAIVTGLQKLWVDSCGCPECLQCLKGLKAIKPGLYEIPRIIPHTKQCSPVNLLNMLVHKLVALRGHVQLAYDARVLTPDFHEIPDLDDSDAVFARTLLAALFHLNMFFILKDYITQDSMSLKQALSGHWMSATGNPLPAAPETLRDYLEAFRNSDNHFYLPTTGPLNTFQFPEELLGRVVVIDSSLCAASHVQDVITHGVGAGVPR
Gene ID
Swiss Prot
SynonymsTegument protein BRRF2; BRRF2
Calculated MW56KDa
Observed MW72kDa
ReactivityEpstein-Barr virus
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2500 - 1:10000
  • IF/ICC 1:100 - 1:400
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293F transfected with BRRF2
Cellular locationVirion tegument.

Documents

Certificate of Compliance

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    ABclonal:Western blot - BRRF2 Rabbit mAb (A23835)}

    Western blot - BRRF2 Rabbit mAb (A23835)

    Western blot analysis of lysates from wild type (WT) and 293F cells transfected with BRRF2 using BRRF2 Rabbit mAb (A23835) at 1:2500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 0.5s.
    ABclonal:Immunofluorescence - BRRF2 Rabbit mAb (A23835)}

    Immunofluorescence - BRRF2 Rabbit mAb (A23835)

    Immunofluorescence analysis of 293F-BRRF2 and 293F cells using BRRF2 Rabbit mAb (A23835) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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