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BRRF2 Rabbit pAb (A22866)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - BRRF2 Rabbit pAb (A22866)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BRRF2 using BRRF2 Rabbit pAb (A22866) at 1:1000 dilution. 
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameBRRF2 Rabbit pAb
Catalog No.A22866
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
BRRF2 is a capsid protein, but it can be secreted into the bloodstream, giving it the opportunity to be a diagnostic or immunotherapy target.The Epstein–Barr virus BRRF2 gene product is involved in viral progeny production .
ImmunogenRecombinant protein of epstein-Barr virus (strain B95-8) (HHV-4) BRRF2.
SequenceMSGQQRGSVILVPEHLAGALTKLMSDFITGQDVTLSGGNIAVKIRDAINQTPGGGDVAILSSLFALWNALPTSGRQSSRDDLIPAAVQALTTAHNLCLGVIPGETSHKDTPESLLRAIVTGLQKLWVDSCGCPECLQCLKGLKAIKPGLYEIPRIIPHTKQCSPVNLLNMLVHKLVALRGHVQLAYDARVLTPDFHEIPDLDDSDAVFARTLLAALFHLNMFFILKDYITQDSMSLKQALSGHWMSATGNPLPAAPETLRDYLEAFRNSDNHFYLPTTGPLNTFQFPEELLGRVVVIDSSLCAASHVQDVITHGVGAGVPR
Gene ID
Swiss Prot
SynonymsTegument protein BRRF2; BRRF2
Calculated MW56kDa
Observed MW100kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T transfected with BRRF2
Cellular locationVirion tegument.

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    ABclonal:Western blot - BRRF2 Rabbit pAb (A22866)}

    Western blot - BRRF2 Rabbit pAb (A22866)

    Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BRRF2 using BRRF2 Rabbit pAb (A22866) at 1:1000 dilution. 
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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