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BST2 Rabbit mAb (A23445)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BST2 Rabbit mAb (A23445)

Western blot analysis of lysates from Mouse liver, using BST2 Rabbit mAb (A23445) at 1:30000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

ABclonal:Immunohistochemistry - BST2 Rabbit mAb (A23445)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using BST2 Rabbit mAb (A23445) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BST2 Rabbit mAb (A23445)

Immunohistochemistry analysis of paraffin-embedded Human spleen using BST2 Rabbit mAb (A23445) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - BST2 Rabbit mAb (A23445)

Confocal imaging of HeLa cells using BST2 Rabbit mAb (A23445, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameBST2 Rabbit mAb
Catalog No.A23445
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC56688
Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 49-160 of human BST2. (NP_004326.1).
SequenceNSEACRDGLRAVMECRNVTHLLQQELTEAQKGFQDVEAQAATCNHTVMALMASLDAEKAQGQKKVEELEGEITTLNHKLQDASAEVERLRRENQVLSVRIADKKYYPSSQDS
Gene ID
Swiss Prot
SynonymsCD317; HM1.24; TETHERIN; BST2
Calculated MW20kDa
Observed MW28-40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:30000
  • IHC-P 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse liver
Cellular locationApical cell membrane, Cell membrane, Cytoplasm, GPI-anchor, Golgi apparatus, Late endosome, Lipid-anchor, Membrane raft, Single-pass type II membrane protein, trans-Golgi network
Customer validation

IF(Mus musculus)

Documents

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ABclonal:Western blot - BST2 Rabbit mAb (A23445)}

Western blot - BST2 Rabbit mAb (A23445)

Western blot analysis of lysates from Mouse liver, using BST2 Rabbit mAb (A23445) at 1:30000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.
ABclonal:Immunohistochemistry - BST2 Rabbit mAb (A23445)}

Immunohistochemistry - BST2 Rabbit mAb (A23445)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using BST2 Rabbit mAb (A23445) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - BST2 Rabbit mAb (A23445)}

Immunohistochemistry - BST2 Rabbit mAb (A23445)

Immunohistochemistry analysis of paraffin-embedded Human spleen using BST2 Rabbit mAb (A23445) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - BST2 Rabbit mAb (A23445)}

Immunofluorescence - BST2 Rabbit mAb (A23445)

Confocal imaging of HeLa cells using BST2 Rabbit mAb (A23445, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

Secondary Antibodies (26)