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BST2 Rabbit pAb (A12315)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - BST2 Rabbit pAb (A12315)

Western blot analysis of various lysates using BST2 Rabbit pAb (A12315) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - BST2 Rabbit pAb (A12315)

Immunohistochemistry analysis of paraffin-embedded Human lung using BST2 Rabbit pAb (A12315) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BST2 Rabbit pAb (A12315)

Immunohistochemistry analysis of paraffin-embedded Human spleen using BST2 Rabbit pAb (A12315) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - BST2 Rabbit pAb (A12315)

Immunofluorescence analysis of HeLa cells using BST2 Rabbit pAb (A12315) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBST2 Rabbit pAb
Catalog No.A12315
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 49-161 of human BST2 (NP_004326.1).
SequenceNSEACRDGLRAVMECRNVTHLLQQELTEAQKGFQDVEAQAATCNHTVMALMASLDAEKAQGQKKVEELEGEITTLNHKLQDASAEVERLRRENQVLSVRIADKKYYPSSQDSS
Gene ID
Swiss Prot
SynonymsCD317; HM1.24; TETHERIN; BST2
Calculated MW20kDa
Observed MW35-40kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesLO2, HeLa, Mouse heart
Cellular locationApical cell membrane, Cell membrane, Cytoplasm, GPI-anchor, Golgi apparatus, Late endosome, Lipid-anchor, Membrane raft, Single-pass type II membrane protein, trans-Golgi network.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - BST2 Rabbit pAb (A12315)}

Western blot - BST2 Rabbit pAb (A12315)

Western blot analysis of various lysates using BST2 Rabbit pAb (A12315) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - BST2 Rabbit pAb (A12315)}

Immunohistochemistry - BST2 Rabbit pAb (A12315)

Immunohistochemistry analysis of paraffin-embedded Human lung using BST2 Rabbit pAb (A12315) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - BST2 Rabbit pAb (A12315)}

Immunohistochemistry - BST2 Rabbit pAb (A12315)

Immunohistochemistry analysis of paraffin-embedded Human spleen using BST2 Rabbit pAb (A12315) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - BST2 Rabbit pAb (A12315)}

Immunofluorescence - BST2 Rabbit pAb (A12315)

Immunofluorescence analysis of HeLa cells using BST2 Rabbit pAb (A12315) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (6)

Secondary Antibodies (26)