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BiP/GRP78 Rabbit pAb (A11366)

Reviews (2)Publications (11) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BiP/GRP78 Rabbit pAb (A11366)

Western blot analysis of various lysates using BiP/GRP78 Rabbit pAb (A11366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - BiP/GRP78 Rabbit pAb (A11366)

Immunohistochemistry analysis of paraffin-embedded mouse brain using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - BiP/GRP78 Rabbit pAb (A11366)

Immunohistochemistry analysis of paraffin-embedded rat brain using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)

Immunofluorescence analysis of NIH/3T3 cells using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)

Immunofluorescence analysis of PC-12 cells using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)

Immunofluorescence analysis of U2OS cells using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for BiP/GRP78 Rabbit pAb(A11366)
Review
ABclonal: review for BiP/GRP78 Rabbit pAb(A11366)

Overview

Product nameBiP/GRP78 Rabbit pAb
Catalog No.A11366
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. This protein localizes to the lumen of the endoplasmic reticulum (ER) where it operates as a typical HSP70 chaperone involved in the folding and assembly of proteins in the ER and is a master regulator of ER homeostasis. During cellular stress, as during viral infection or tumorogenesis, this protein interacts with the transmembrane stress sensor proteins PERK (protein kinase R-like endoplasmic reticulum kinase), IRE1 (inositol-requiring kinase 1), and ATF6 (activating transcription factor 6) where it acts as a repressor of the unfolded protein response (UPR) and also plays a role in cellular apoptosis and senescence. Elevated expression and atypical translocation of this protein to the cell surface has been reported in viral infections and some types of cancer cells. At the cell surface this protein may facilitate viral attachment and entry to host cells. This gene is a therapeutic target for the treatment of coronavirus diseases and chemoresistant cancers.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 550-654 of human BiP/GRP78 (NP_005338.1).
SequenceEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL
Gene ID
Swiss Prot
SynonymsBIP; GRP78; HEL-S-89n; BiP/GRP78
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, U-87MG, Mouse liver, Rat liver, Rat testis
Cellular locationCytoplasm, Endoplasmic reticulum lumen, Melanosome
Customer validation

(Human renal epithelial cell line, Pheochromocytoma in mice)

WB(Homo sapiens)

IHC(Rattus norvegicus, Homo sapiens, Mus musculus)

WB(Homo sapiens,Mus musculus)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - BiP/GRP78 Rabbit pAb (A11366)}

Western blot - BiP/GRP78 Rabbit pAb (A11366)

Western blot analysis of various lysates using BiP/GRP78 Rabbit pAb (A11366) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - BiP/GRP78 Rabbit pAb (A11366)}

Immunohistochemistry - BiP/GRP78 Rabbit pAb (A11366)

Immunohistochemistry analysis of paraffin-embedded mouse brain using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - BiP/GRP78 Rabbit pAb (A11366)}

Immunohistochemistry - BiP/GRP78 Rabbit pAb (A11366)

Immunohistochemistry analysis of paraffin-embedded rat brain using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)}

Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)

Immunofluorescence analysis of NIH/3T3 cells using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)}

Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)

Immunofluorescence analysis of PC-12 cells using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)}

Immunofluorescence - BiP/GRP78 Rabbit pAb (A11366)

Immunofluorescence analysis of U2OS cells using BiP/GRP78 Rabbit pAb (A11366) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)