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CCNB1IP1 Rabbit pAb (A16693)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - CCNB1IP1 Rabbit pAb (A16693)

Western blot analysis of lysates from mouse heart, using CCNB1IP1 Rabbit pAb (A16693) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameCCNB1IP1 Rabbit pAb
Catalog No.A16693
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
HEI10 is a member of the E3 ubiquitin ligase family and functions in progression of the cell cycle through G(2)/M.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-50 of human CCNB1IP1 (NP_878269.1).
SequenceMSLCEDMLLCNYRKCRIKLSGYAWVTACSHIFCDQHGSGEFSRSPAICPA
Gene ID
Swiss Prot
SynonymsHEI10; C14orf18; CCNB1IP1
Calculated MW32kDa
Observed MW31kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesmouse heart
Cellular location
Customer validation

IF(Mus musculus)

WB(Sus scrofa)

    ABclonal:Western blot - CCNB1IP1 Rabbit pAb (A16693)}

    Western blot - CCNB1IP1 Rabbit pAb (A16693)

    Western blot analysis of lysates from mouse heart, using CCNB1IP1 Rabbit pAb (A16693) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16693? Please let us know so that we can cite the reference in this datasheet.

    Secondary Antibodies (26)