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CD10/MME Rabbit mAb (A22179)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - CD10/MME Rabbit mAb (A22179)

Western blot analysis of various lysates, using CD10/MME Rabbit mAb (A22179) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:200000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CD10/MME Rabbit mAb (A22179)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD10/MME Rabbit mAb (A22179) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD10/MME Rabbit mAb (A22179)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD10/MME Rabbit mAb (A22179) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameCD10/MME Rabbit mAb
Catalog No.A22179
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55335
The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 52-750 of human CD10/MME (NP_000893.2).
SequenceYDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
Gene ID
Swiss Prot
SynonymsNEP; SFE; CD10; CALLA; CMT2T; SCA43; CD10/MME
Calculated MW86kDa
Observed MW100kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesDaudi, Raji
Cellular locationCell membrane, Single-pass type II membrane protein.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CD10/MME Rabbit mAb (A22179)}

Western blot - CD10/MME Rabbit mAb (A22179)

Western blot analysis of various lysates, using CD10/MME Rabbit mAb (A22179) at1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:200000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - CD10/MME Rabbit mAb (A22179)}

Immunohistochemistry - CD10/MME Rabbit mAb (A22179)

Immunohistochemistry analysis of paraffin-embedded Human liver using CD10/MME Rabbit mAb (A22179) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD10/MME Rabbit mAb (A22179)}

Immunohistochemistry - CD10/MME Rabbit mAb (A22179)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using CD10/MME Rabbit mAb (A22179) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A22179? Please let us know so that we can cite the reference in this datasheet.

항체 (8)

ELISA 키트 (1)

재조합 단백질 (1)

Secondary Antibodies (26)