제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Human CD10 mAb (A25723)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 A549 cells (negative control, left) and U-138MG cells (right) were surface-stained with PE Rabbit anti-Human CD10 mAb (A25723, 5 μl/Test, orange line) or PE Rabbit IgG isotype control(A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 U-138MG cells were surface-stained with PE Rabbit IgG isotype control(A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD10 mAb (A25723, 5 μl/Test, right).

Flow cytometry: 1X10^6 A549 cells (negative control, left) and Daudi cells (right) were surface-stained with PE Rabbit anti-Human CD10 mAb (A25723, 5 μl/Test, orange line) or PE Rabbit IgG isotype control(A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Daudi cells were surface-stained with PE Rabbit IgG isotype control(A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD10 mAb (A25723, 5 μl/Test, right).

Flow cytometry:1X10^6 Human Neutrophile granulocytes were surface-stained with APC Rabbit anti-Human CD66b mAb (A26616, 5 μl/Test) and PE Rabbit anti-Human CD10 mAb (A25723, 5 μl/Test, right) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, left).

Overview

Product name	PE Rabbit anti-Human CD10 mAb
Catalog No.	A25723
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55330-PE

Background

The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 52-750 of human CD10 (NP_000893.2).
Sequence	YDDGICKSSDCIKSAARLIQNMDATTEPCTDFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKALYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQIQNNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGLGQAYRAYQNYIKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW
Gene ID
Swiss Prot
Synonyms	NEP; SFE; CD10; CALLA; CMT2T; SCA43
Calculated MW	86kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type II membrane protein.

Documents

Certificate of Compliance

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Research Area

PE Rabbit anti-Human CD10 mAb images

Flow CytoMetry - PE Rabbit anti-Human CD10 mAb (A25723)

Flow cytometry: 1X10^6 U-138MG cells were surface-stained with PE Rabbit IgG isotype control(A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD10 mAb (A25723, 5 μl/Test, right).

Flow CytoMetry - PE Rabbit anti-Human CD10 mAb (A25723)

Flow cytometry: 1X10^6 Daudi cells were surface-stained with PE Rabbit IgG isotype control(A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD10 mAb (A25723, 5 μl/Test, right).

Flow CytoMetry - PE Rabbit anti-Human CD10 mAb (A25723)

* For research use only. Not for therapeutic or diagnostic purposes.