제품 > 항체 > 단일클론항체(mAb)

CD10 Rabbit mAb (A25707)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CD10 using CD10 Rabbit mAb (A25707) at 1:3000 dilution incubated overnight at 4°C.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 60 s.

Western blot analysis of lysates from Mouse kidney using CD10 Rabbit mAb (A25707) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 30 s.

ABclonal:Flow CytoMetry - CD10 Rabbit mAb (A25707)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T cells (right) were surface-stained with CD10 Rabbit mAb (A25707, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line). followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD10 Rabbit mAb (A25707, 2 μg/mL, right), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining.

Overview

Product name	CD10 Rabbit mAb
Catalog No.	A25707
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC67050

Background

Enables peptidase activity. Involved in amyloid-beta clearance; positive regulation of long-term synaptic potentiation; and sensory perception of pain. Acts upstream of or within amyloid-beta metabolic process. Located in several cellular components, including dendrite; neuron projection terminus; and synaptic vesicle. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; liver and biliary system; and mammary gland. Used to study Alzheimer's disease. Human ortholog(s) of this gene implicated in Alzheimer's disease; Charcot-Marie-Tooth disease axonal type 2T; cerebellar ataxia type 43; cerebral amyloid angiopathy; and membranous glomerulonephritis. Orthologous to human MME (membrane metalloendopeptidase).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 52-750 of mouse CD10 (NP_032630.2).
Sequence	YDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSSRYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKLLPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIHFDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKVMELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTVNINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSYMNPERKCRVW
Gene ID
Swiss Prot
Synonyms	Mme
Calculated MW	86kDa
Observed MW	100kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 FC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	293F transfected with CD10 (Mouse), Mouse kidney
Cellular location	Cell membrane, Single-pass type II membrane protein