제품 > 항체 > 다중클론항체(pAb)

CD112 Rabbit pAb (A23657)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using CD112 Rabbit pAb (A23657) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product name	CD112 Rabbit pAb
Catalog No.	A23657
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables cell adhesion molecule binding activity and protein homodimerization activity. Involved in homophilic cell adhesion via plasma membrane adhesion molecules. Acts upstream of or within several processes, including establishment of mitochondrion localization; sperm mitochondrion organization; and spermatid development. Located in cell-cell contact zone; plasma membrane; and zonula adherens. Is expressed in brain; cochlea; enamel organ; male reproductive system; and primitive collecting duct. Human ortholog(s) of this gene implicated in Alzheimer's disease and multiple sclerosis. Orthologous to human NECTIN2 (nectin cell adhesion molecule 2).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 32-345 of mouse CD112. (NP_033016.3).
Sequence	QDVRVRVLPEVRGRLGGTVELPCHLLPPTTERVSQVTWQRLDGTVVAAFHPSFGVDFPNSQFSKDRLSFVRARPETNADLRDATLAFRGLRVEDEGNYTCEFATFPNGTRRGVTWLRVIAQPENHAEAQEVTIGPQSVAVARCVSTGGRPPARITWISSLGGEAKDTQEPGIQAGTVTIISRYSLVPVGRADGVKVTCRVEHESFEEPILLPVTLSVRYPPEVSISGYDDNWYLGRSEAILTCDVRSNPEPTDYDWSTTSGVFPASAVAQGSQLLVHSVDRMVNTTFICTATNAVGTGRAEQVILVRESPSTAG
Gene ID
Swiss Prot
Synonyms	MPH; Pvr; Pvs; Cd112; Pvrl2; nectin-2; CD112
Calculated MW	57kDa
Observed MW	65-85kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Molt-4, C2C12
Cellular location